Generator

Part:BBa_K990003:Design

Designed by: Fatma Betul CEVIK   Group: iGEM13_NGSS_AEI_TURKEY   (2013-06-21)


HO1+PcyA+pOmpC+Amp Resistance


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2859
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2860
    Illegal PstI site found at 2874
    Illegal NotI site found at 7
    Illegal NotI site found at 2867
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 1647
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2860
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2860
    Illegal PstI site found at 2874
    Illegal NgoMIV site found at 1200
    Illegal NgoMIV site found at 2566
    Illegal AgeI site found at 2474
    Illegal AgeI site found at 2681
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amp resistance gene got from NCBI


Source

iGEM parts registry. We looked M30109, I15008, I15009, and I15010.

References